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. 2011 Jul 19;30(16):3430–3441. doi: 10.1038/emboj.2011.240

Figure 2.

Figure 2

Leaf development shows enhanced endoreduplication and slowed down cell-cycle progression in cyca2 triple mutants. (A) Distribution of nuclear ploidy in mature primary leaves of WT, cyca2;124, cyca2;134 and cyca2;234. Triple mutants cyca2;134 and cyca2;234 show highest ploidy levels. (B) Pavement cell size in mature primary leaves of WT, cyca2;124, cyca2;134 and cyca2;234. Yellow overlays highlight representative cells. (C) Kinematic analysis reveals a slowdown in cell division rates in developing primary leaves cyca2;234 compared with WT. (D) Evolution of ploidy levels during the development of WT and cyca2;234 primary leaves. In early stages, WT has predominantly 2C nuclei and a low 4C fraction. Later, the 2C fraction drops rapidly, while higher ploidy fractions increase until ∼16 days after stratification. In cyca2;234, the 2C fraction is already low at the earliest stage analysed, while the 4C fraction is already high and even a small fraction 8C nuclei can be detected. At later stages, higher ploidy fractions continue to increase, and do not saturate within the time frame of our analysis. Data are represented as mean±s.e.