Figure 5.
CYCA2;2, CYCA2;3, CYCA2;4 and CDKB1;1 genes synergistically promote guard mother cell symmetric division. (A) Micrographs of WT, cdkb1;1, cyca2;234 and quadruple cyca2;234/cdkb1;1 cotyledons of 4-day-old seedlings. Cell walls in 4-day-old developing cotyledons were visualized using propidium iodide and laser scanning confocal microscopy. False colouring highlights stomatal complexes: blue=normal stoma; yellow=SGC. (B) Quantification of stomatal phenotypes. Quadruple cyca2;234 cdkb1;1 mutant displays more SGCs than cyca2;234. (Fisher’s exact test, P=0.0004).