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. 2011 Jul 19;30(16):3353–3367. doi: 10.1038/emboj.2011.241

Figure 1.

Figure 1

A genetic screen for TBZ- and benomyl-sensitive mutants with the histone-GLibrary (Matsubara et al, 2007; Sakamoto et al, 2009). (A) Sensitivity to microtubule-depolymerizing agents was determined by dropping three-fold serial dilutions of histone point mutants on the FY406 or MSY748 background onto SC agar plates containing 25 μg/ml thiabendazole (TBZ) or 10 μg/ml benomyl diluted in 0.125% dimethyl sulphoxide (DMSO). The plates were incubated at 30 °C for 3 days. (B) The position of histone residues that conferred TBZ/benomyl sensitivity on the electrostatic surface of the nucleosome core (PDB ID: 1ID3; White et al, 2001) looking down from the DNA superhelix axis. The positions of each residue, which is visible or not directly visible, are indicated by solid and dashed lines, respectively. Classification of TBS-I, -II, and -III residues is listed in Table I. (C, D) Side (C) and top (D) view of the same structure shown in (B).