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. 2011 Jul 19;30(16):3353–3367. doi: 10.1038/emboj.2011.241

Figure 5.

Figure 5

Sgo1 is a multi-copy suppressor of histones H2A and H3 point mutations in TBS-I and -II. (A) Illustration of an experimental approach to investigate the functional interaction between Sgo1 and TBS-I, -II, and -III residues (Figure 1B–D; Table I). (B) The sensitivity of histone H2A point mutants in the C-terminal region to 25 μg/ml TBZ and 10 μg/ml benomyl was determined using three-fold serial dilutions of cells. Each strain was transformed with a YEplac195 multi-copy vector (URA3 marker, 2 μ) containing the SGO1 gene. The plates were incubated for 3 days at 25 °C. (C–E) The sensitivity of representative TBS-I (C), TBS-II (D), and TBS-III (E) mutants to TBZ and benomyl was determined using three-fold serial dilutions of cells as described in (B).