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. 2011 Jul 26;30(16):3397–3415. doi: 10.1038/emboj.2011.248

Figure 6.

Figure 6

p53 knockdown by p53 siRNA up-regulated BNIP3 expression under normoxia and hypoxia in HCT116 cells. (A, C) the expression level of endogenous p53 protein was knocked down by p53 siRNA treatment (middle panels in A, C) and the expression level of endogenous BNIP3 protein was increased (upper panels in A, C) after p53 knockdown under normoxia (A) and hypoxia (C), as revealed by western blot using monoclonal antibodies against p53 and BNIP3. β-Actin was used as the loading control (bottom panels in A, C). The relative fold of p53 protein was marked underlined. (B, D) BNIP3 mRNA was up-regulated by p53 knockdown under normoxic (B) and hypoxic (D) conditions as revealed by semi-quantitative RT–PCR (P=0.01 and P=0.0001, respectively). The primers to amplify human 18S RNA was used as an internal control. Data of RT–PCR analysis are reported as mean±s.d. of three independent experiments performed in triplicate; the statistical analysis was performed using GraphPad Prism 5.0 (t-tests).