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. 2011 Jul 26;30(16):3397–3415. doi: 10.1038/emboj.2011.248

Figure 7.

Figure 7

p53 overexpression suppressed BNIP3 expression under normoxia and hypoxia in HCT116 cells. (A, C) The expression level of endogenous BNIP3 protein was reduced by p53 overexpression under normoxia (A) and hypoxia (C), as revealed by western blot using monoclonal antibodies against BNIP3, HA tag and p53. β-Actin was used as the loading control. (B, D) The expression of endogenous BNIP3 mRNA was suppressed by p53 overexpression under normoxia (B) and hypoxia (D) as revealed by semi-quantitative RT–PCR (P=0.0331 and P=0.0005, respectively). The primers to amplify human 18S RNA was used as an internal control. Data of RT–PCR analysis are reported as mean±s.d. of three independent experiments performed in triplicate; the statistical analysis was performed using GraphPad Prism 5.0 (t-tests).