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. 2011 Jul 29;30(16):3242–3258. doi: 10.1038/emboj.2011.257

Figure 4.

Figure 4

V-ATPase regulation by mTORC1 requires Tfeb and Tfeb is an important mediator of the changes in gene expression induced by mTORC1. (A) qRT–PCR of MEFs of the indicated genotypes stably transduced with the indicated shRNA vectors; each Tfeb shRNA (A, B) was compared with the scrambled (Sc) control by an unpaired t-test (n=3). Error bars represent s.e.m.; *P<0.05; ***P<0.001. (B) Western blot of serum-starved MEFs of the indicated genotypes. (C) Number of probes (genes) corresponding to the different patterns upregulated under one condition compared with the other three by t-tests (Q<0.05 after FDR correction) in Illumina microarrays. Data are shown also incorporating a fold change criterion (>1.5-fold; FC>1.5). (D) Heatmap representation of normalized expression values for all 1418 significant LLHL probes with FC>1.5 in scrambled shRNA transduced MEFs compared with Tfeb shRNA transduced MEFs. Box shows probes for which mean expression levels are reduced by >20% following Tfeb knockdown in Tsc2−/− MEFs. (E) qRT–PCR of Tsc2+/+ and Tsc2−/− MEFs stably transduced with the indicated shRNA vectors and treated (or not) with rapamycin (n=3). Error bars represent s.e.m.; NS, non-significant; **P<0.01; ***P<0.001.