Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Jul 29;30(16):3242–3258. doi: 10.1038/emboj.2011.257

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2011, European Molecular Biology Organization

This is an open-access article distributed under the terms of the Creative Commons Attribution Noncommercial Share Alike 3.0 Unported License, which allows readers to alter, transform, or build upon the article and then distribute the resulting work under the same or similar license to this one. The work must be attributed back to the original author and commercial use is not permitted without specific permission.

PMC Copyright notice

Coordinated regulation of Tfeb phosphorylation and nuclear localization by the Tsc1/Tsc2 complex and rapamycin. (A, B) Western blot of MEFs of the indicated genotypes in 0.1% serum treated or not with rapamycin for 24 h (as in Figure 1B). (C) Western blot of serum-starved (0.1% serum) Tsc2^−/− cells treated with rapamycin for the indicated times. (D) Western blot of Tsc2^+/+ and Tsc2^−/− cells under serum starvation conditions treated (or not) with rapamycin and subsequently incubated, as stated, with λ phosphatase. (E) Western blot of serum-starved Tsc2^+/+ and Tsc2^−/− cells treated (or not) with rapamycin and subsequently fractionated into nuclear [N] or cytoplasmic [C] fractions. (F) Western blots of nuclear versus cytoplasmic fractions from serum-starved Tsc2^−/− cells incubated as indicated with λ phosphatase. (G) Western blot of whole-cell lysates (WCL) or nuclei from serum-starved Tsc2^−/− cells treated with rapamycin for the indicated periods of time.