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Methods for generating gradient hydrogels. A: Gradient generation by (i) a source-sink diffusion device, (ii) a tree-like microfluidic gradient generator, (iii) mixing the input streams from syringe pumps, and (iv) microfluidic convection. B: Cross-linking by photopolymerisation using (i) a normal setup, (ii) a gradient mask, (iii) a sliding mask, and cross-linking by (iv) chemicals, and (v) heat.
