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. Author manuscript; available in PMC: 2011 Aug 24.
Published in final edited form as: Can J Chem Eng. 2010 Dec;88(6):899–911. doi: 10.1002/cjce.20411

Figure 6.

Figure 6

Differentiation of fibroblasts into myofibroblasts mediated by elasticity gradients in PEG hydrogels. Valvular interstitial cells (VICs) cultured on elasticity gradient hydrogels were immunostained for αSMA (green), an indicator of differentiation to myofibroblasts, as well as F-actin (red) and nuclei (blue). Images were taken at different representative positions along the gradient (elastic moduli noted on image). By day 3, a higher myofibroblastic differentiation was observed in cells on the high modulus side of the gradient (Kloxin et al., 2010).