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. Author manuscript; available in PMC: 2012 Aug 5.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Jun 25;411(3):490–495. doi: 10.1016/j.bbrc.2011.06.132

Figure 1.

Figure 1

1A. Immunoblots showing the knock-down of CYPOR and a concurrent reduction in the Connexin 43 (Cx43) levels in stably generated CYPOR knock-down 2T3 and MG63 cell lines. (shRNA- cells treated with POR shRNA vector, Ctrl – cells with cloning vector, Scl – Scrambled shRNA vector). 1B. Immunofluorescence images of gap junction plaques on the cell membranes of MG63 cells and 2T3 cells. The CYPOR knock-down MG63 and 2T3 cells show decreased membrane-localized Cx43 gap junction plaques and intracellular Cx43 compared to the wild type cells (arrows)