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. 2011 Aug 24;6(8):e22625. doi: 10.1371/journal.pone.0022625

Figure 8. CypE has no effect on NP-RNA binding.

Figure 8

(A) Purified NP and CypE proteins were ≥95% pure according to SDS-PAGE. Purified NP (1, 5, or 10 µg) was incubated with poly(U) agarose in 200 µl buffer, respectively, and blank agarose without poly(U) coupling was incubated with 10 µg NP as a negative control. (B) NP was incubated with different doses of CypE for 4 h at 4°C in 200 µl buffer. Then, poly(U)-agarose was incubated with the mixtures for 15 min at 4°C. His-NP bound to the agarose was detected by western blotting using an anti-His monoclonal antibody.