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. 2011 Aug 24;6(8):e23414. doi: 10.1371/journal.pone.0023414

Figure 1. FGF or EGF priming exhibits different temporal patterns of signal activation in hNSCs.

Figure 1

(A–B) Representative phase contrast microscope images show hNSCs primed in either FL (A) or ELL (B) for 4 days. Scale bar = 25 µm. (C–D) Temporal patterns of signal phosphorylations in FL-primed vs. ELL -primed hNSCs at different time points assessed by Western blot analyses. Densitometric values, depicted as a ratio of the phosphorylated form over total amount of the specific protein, are plotted for phospho-Akt Ser473 (C) and phospho-GSK3β (Ser 9) (D). Data are presented as mean ± SEM (n = 3); * p<0.05, two-way ANOVA.