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Emission spectra (Perkin-Elmer LS50B; Ex; 338 nm; Em: 400-650 nm) of 0.125 μM PDMPO in 20 mM PIPES solutions at pH 7 and; a. with or without 5% w/v callose; b. 2 mM Si(OH)4 with or without 5% w/v callose; c. 7 mM Si(OH)4 with or without 5% w/v callose.
