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. 2011 Jul 7;11:44. doi: 10.1186/1471-213X-11-44

Figure 1.

Figure 1

Recombinant BMP-2 had neither inhibitory nor proliferative effects on cultured tongue. Gross morphology of E13 tongues cultured for 8 days in BGJb medium containing vehicle (A) and 4 μg/ml of human recombinant BMP-2 (B). B used the same magnification as A. Expression level of GAPDH mRNA in E13 tongues cultured for 8 days in BGJb medium containing vehicle and 4 μg/ml of human recombinant BMP-2 (C). The longitudinal axis in C represents the quantity of GAPDH mRNA contained in one cultured tongue. Expression levels of cyclin D1 (D) and CDK4 (E) mRNAs in E13 tongues cultured for 8 days in BGJb medium containing vehicle and 4 μg/ml of human recombinant BMP-2. The longitudinal axis in D and E represents the ratio of target gene mRNA relative to GAPDH mRNA. Each column and vertical bar represent the mean + 1SD of six cultured tongues.