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. 2011 Jul 7;11:44. doi: 10.1186/1471-213X-11-44

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Copyright ©2011 Aoyama et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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BMP-2 siRNA induced the expressions of myogenin and fMyHC in cultured tongue. Immunostaining images for myogenin (A, B) and fMyHC (C, D) in the middle portion of E13 tongues cultured for 8 days in BGJb medium containing 250 nM of the NTC (A, C) and BMP-2 siRNA (B, D). Brown color indicates immunostaining image for myogenin and fMyHC. Arrows in A and B indicate myogenin-positive nuclei. Western blotting pattern of myogenin, fMyHC, and βIII-tubulin in the whole portion of E13 tongues cultured for 8 days in BGJb medium containing the NTC and BMP-2 siRNA (E).