Table 4.
Sequences of primer specific for target genes
| Genes | ||
|---|---|---|
| MyoD | Forward | 5'-GAC GGC TCT CTC TGC TCC-3' |
| Reverse | 5'-AAG TGT GCG TGC TCC TCC-3' | |
| Myogenin | Forward | 5'-GAG CGC GAT CTC CGC TAC AGA GG-3' |
| Reverse | 5'-CTG GCT TGT GGC AGC CCA GG-3' | |
| MCK | Forward | 5'-CAA TAA GCT TCG CGA TAA GGA G-3' |
| Reverse | 5'-GAT GGG ATC AAA CAG GTC CTT G-3' | |
| Id1 | Forward | 5'-ACG ACA TGA ACG GCT GCT ACT-3' |
| Reverse | 5'-GCT CAC TTT GCG GTT CTG G-3' | |
| Runx2 | Forward | 5'-TTT AGG GCG CAT TCC TCA TC-3' |
| Reverse | 5'-TGT CCT TGT GGA TTA AAA GGA CTT G-3' | |
| Osteocalcin | Forward | 5'-TGC TTG TGA CGA GCT ATC AG-3' |
| Reverse | 5'-GAG GAC AGG GAG GAT CAA GT-3' | |
| ALP | Forward | 5'-CAC GGG CAC CAT GAA GGA AAA G-3' |
| Reverse | 5'-TGG CGC AGG GGC ACA GGA GAC T-3' | |
| Collagen II | Forward | 5'-ACT GGT AAG TGG GGC AAG AC-3' |
| Reverse | 5'-CCA CAC CAA ATT CCT GTT CA-3' | |
| Collagen X | Forward | 5'-TGG GTA GGC CTG TAT AAA GAA CGG-3' |
| Reverse | 5'-CAT GGG AGC CAC TAG GAA TCC TGA GA-3' | |
| Cyclin D1 | Forward | 5'-AGG CGG ATG AGA ACA AGC AGA-3' |
| Reverse | 5'-CAG GCT TGA CTC CAG AAG GG-3' | |
| CDK4 | Forward | 5'-ACG CCT GTG GTG GTT ACG CT-3' |
| Reverse | 5'-CCA TCT CTG GCA CCA CTG AC-3' | |
| BMP-2 | Forward | 5'-GTG GTG GAA GTG GCC CAC TT-3' |
| Reverse | 5'-CTG TTT GTG TTT GGC TTG ACG-3' | |
| BMP-4 | Forward | 5'-TCC TGG TAA CCG AAT GCT GAT-3' |
| Reverse | 5'-GCT GCT GAG GTT GAA GAG GAA-3' | |
| GAPDH | Forward | 5'-GAT GCT GGT GCT GAG TAT GTC G-3' |
| Reverse | 5'-GTG GTG CAG GAT GCA TTG CTG A-3' |