Table 1.
Identified polymorphisms in porcine TFRC, ACK1, MUC4, MUC20 and KIAA0226 genes
| Gene | No. | Base | Consequence |
|---|---|---|---|
| TFRC (100.8 Mb) | 1 | G→A (E4) | Ala→Thr |
| 2 | T→C (E10) | Silent | |
| 3 | G→T (I11) | 10 bp before branch point | |
| ACK1 (100.9 Mb) | 4 | C→G (E1) | Pro→Ala |
| 5 | T→C (E2) | Silent | |
| 6 | G→T (E2) | Silent | |
| 7 | C→T (E3) | Silent | |
| 8 | A→C (E7) | Silent | |
| 9 | C→T (E7) | Silent | |
| 10 | T→C (E9) | Silent | |
| 11 | C→T (E9) | Silent | |
| 12 | G→A (E10) | Silent | |
| 13 | C→A (E12) | Pro→His | |
| 14 | A→G (E12) | Silent | |
| 15 | G→A (E12) | Silent | |
| 16 | G→A (E12) | Val→Met | |
| 17 | A→G (E12) | Silent | |
| 18 | G→A (E12) | Arg→His | |
| 19 | G→C (E12) | Ser→Thr | |
| 20 | C→G (I14) | 5 bases before exon 15 | |
| MUC4 (101.0 Mb) | 21 | C→A (E12) | Silent |
| 22 | C→T (I13) | 4 bases before exon 14 | |
| 23 | C→T (I13) | 16 bases before exon 14 | |
| 24 | C→T (I13) | 28 bases before exon 14 | |
| 25 | C→T (E19) | Silent | |
| 26 | G→A (E24) | Gly→Ser | |
| 27 | T→C (E24) | Silent | |
| 28 | G→A (3UTR) | 187 bases after TGA | |
| 29 | G→A (3UTR) | 259 bases after TGA | |
| MUC20 (101.1 Mb) | 30 | A→C (3UTR) | 201 bases after TGA |
| 31 | C→T (I2) | 58 bases after exon 2 | |
| 32 | C→T (E2) | Silent | |
| 33 | A→T (E1) | Silent | |
| KIAA0226 (101.1 Mb) | 34 | C→T (E20) | Silent |
A total of 34 polymorphisms were identified in the four tested animals (2 resistant Wild Boars and 2 homozygous susceptible Large White sows). Gene names are shown in the first column (gene positions at chromosome 13 in Sscrofa 9 are in brackets), polymorphisms in the second column and E and I in brackets indicate exonic (E) or intronic (I) location of the polymorphisms. The third column shows the nature of the polymorphisms. The six polymorphisms matching the ETEC F4ab/ac genotype perfectly in the four animals are in bold, and the two polymorphisms underlined are also closely associated with the ETEC F4ab/ac status in the additional 42 animals.