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. Author manuscript; available in PMC: 2011 Aug 24.
Published in final edited form as: Dev Biol. 2009 Oct 20;337(1):99–109. doi: 10.1016/j.ydbio.2009.10.014

Fig. 6. Gprk2 promotes Smo phosphorylation.

Fig. 6

(A) Immunoblot analysis of wing disc lysates probed with antibody against Smo. Lysates were prepared from wild-type (w1118), gprk2del1/grpk2KO, and gprk2del1/grpk2del2 larvae cultured at 29°C (B) Wing disc lysates prepared from wild-type or gprk2del1/grpk2KO larvae were treated with (+) or without (−) λ-phosphatase and immunoblotted with antibody against Smo. (C) S2 cells were pretreated with dsRNA targetting gfp (Control) or gprk2, and then incubated for 2 h with control (− Hh) or HhN (+ Hh) conditioned medium. Lysates were separated by SDS-PAGE and immunoblotted with antibodies against Smo (top) and Gprk2 (middle). Samples were run in parallel on a higher percentage gel and reprobed with antibody against α-Tubulin to ensure equal loading (bottom).