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. Author manuscript; available in PMC: 2011 Aug 24.
Published in final edited form as: Dig Dis Sci. 2010 Feb;55(2):484–495. doi: 10.1007/s10620-009-0955-5

Fig. 4.

Fig. 4

High doses of sCD25 induces T cell apoptosis. CD4+CD25− T cells from a normal healthy subject (NHC) were cultured in SFM alone, in SFM with PHA but no sCD25 and with increasing doses of recombinant sCD25 (6,000 and 60,000 pg/ml) in the presence of PHA in order to determine if apoptosis induction can explain the observed sCD25 mediated T cell suppression. Histograms with the mean fluorescence intensity for Annexin V staining are shown for a representative CD4+CD25− T cell experiment. Increasing doses of sCD25 induced apoptosis with the majority of apoptotic cells seen with the highest sCD25 concentration (27.7% with 0 pg/ml of sCD25 vs. 80.6% with 6,000 pg/ml of sCD25 vs. 90.2% with 60,000 pg/ml of sCD25)