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. 2011 Aug 24;6(8):e23878. doi: 10.1371/journal.pone.0023878

Figure 5. Correct localization of ZnT5 requires the region 525–666 according to amino acid numbering of Variant A, containing the histidine-rich segment, plus the C-terminal region specific to variant A or B.

Figure 5

(A) Hela cells expressing transiently a construct of ZnT5 representing the region ‘core’ to both variants (amino acids172–666) with a C-terminal GFP tag. The ER is stained (red) with ER Tracker. Note that localization overlaps with the ER stain, but also includes punctuate structures. Diagram illustrating truncated protein construct 172–666 of ZnT5 shown alongside (B) Hela cells expressing transiently a construct of ZnT5 comprising the region 172 to 524 with a C-terminal GFP tag. In (ii) the ER is stained (red) with ER Tracker. The inset panel shows the Golgi apparatus stained (red) using WGA. Note that the pattern of localization overlaps with but is not restricted to the Golgi apparatus or ER. Diagram illustrating truncated protein construct 172–524 of ZnT5 shown alongside.