Table 1. Primers and cycling parameters for PCR.

Product	Primer sequence	Product Size (bp)	Cycling Parameters
ap-GFPZnT5A	201 GATGGAGGAGAAATACGGCG 220 2496 ATGATGTAAGTACCATCTTTGC 2474	2295	96°C – 30 s, 56°C – 30 s, 72°C – 90 s, 35 cycles
a Variant A cDNA – 3′ end	1942 GCGGGTGGAGGCATGAATGCTA 1963 2496 ATGATGTAAGTACCATCTTTGC 2474	551	95°C – 30 s, 60°C – 30 s, 72°C – 60 s, 25 cycles
a Variant A (172–666)-GFP clone	714 CATGGCTAAAATGGCTGAACAC 735 2199 CTTTTCTAAAGCAATATGTAGTTCT 2175	1485	96°C – 30 s, 56°C – 30 s, 72°C – 90 s, 35 cycles
a Variant A (172–524)-GFP clone	714 CATGGCTAAAATGGCTGAACAC 735 1770 TGTTAACATGTGAGTGTCTAATTC 1747	1056	94°C – 30 s, 57°C – 60 s, 72°C – 90 s, 35 cycles
b Full length variant B cDNA/pZnT5B-GFP	1 ATGGCTAAAATGGCTGAACAC 22 1569 TTTGGTTGTCTGTTTTACTTCCAG 1546	1569	96°C – 30 s, 60°C – 30 s, 72°C – 90 s 35 cycles
b P-GFPZnT5B	1 ATGGCTAAAATGGCTGAACAC 22 1569 TTTGGTTGTCTGTTTTACTTCCAG 1546	1569	96°C – 30 s, 60°C – 30 s, 72°C – 90 s 35 cycles
b Variant B cDNA – 3′ end	1228 GCGGGTGGAGGCATGAATGCTA 1249 1569 TTTGGTTGTCTGTTTTACTTC 1549	341	94°C – 30 s, 60°C – 30 s, 72°C – 60 s, 30 cycles
Variant B (K519E)	1 ATGGCTAAAATGGCTGAACAC 22 1569 TTTGGTTGTCTGTTCTACTTCCA 1547	1569	96°C – 30 s, 60°C – 30 s, 72°C – 90 s 30 cycles

Times are given in the order denaturing, annealing, extension and the number of cycles is indicated.

^aNumbered according to Genbank™ sequence NM_022902.

^bNumbered according to Genbank ™sequence JF802034.