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. 2011 Aug 24;6(8):e23983. doi: 10.1371/journal.pone.0023983

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Khajuria et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Immediately after the larvae developed into second instar, they were individually fed fresh artificial ECB diet containing dsRNA. (A) The expression of OnAPP gene in OnAPP dsRNA and GFP dsRNA treated larvae after 4, 6, and 8 days. Standard errors of the means were determined from three biological replications, each with two technical replications. (B) The expression of OnAPP for six individual midguts after 8 days of dsRNA feeding. (C) Percent mortality in OnAPP dsRNA, GFP dsRNA , and water treated larvae. Standard errors of the means were determined from three replications; each replication consisted of 50 larvae. Different letters represent significant difference with p value ≤0.05.