FIG. 5.

Histological analysis of R-TE-MR retrieved 2 months after implantation. Immunohistological staining with desmin (A: 100×and B: 400×) provides further evidence for the presence of myofibers (black arrowheads) and blood vessels (white arrowheads) inside the TE-MR construct. NF-200 staining (C) indicates the presence of nerves at the interface of the native LD muscle and TE-MR construct. Staining with Von Willebrand factor (vWF) (D) demonstrates the presence of blood vessels (white arrowheads) at the interface of the native LD muscle and TE-MR construct. Black rectangle in A represents the approximate location of B and white rectangle represents approximate location of C. All scale bars=100 μm. Color images available online at www.liebertonline.com/tea