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. 2011 Aug 24;6(8):e24064. doi: 10.1371/journal.pone.0024064

Figure 3. Human SCF (hSCF) does not activate mouse c-KIT.

Figure 3

(A) Endogenous mouse c- kit immunoprecipitated from Melan-A cells stimulated with mouse SCF (mSCF) was autophosphorylated. (B) However, the addition of hSCF to the Melan-A cells did not activate endogenous mouse c-KIT. Immunoblotting with 4G10 antibody was used to detect general tyrosine phosphorylation to assess the c-KIT phosphorylation/ activation.