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. Author manuscript; available in PMC: 2013 Jan 1.
Published in final edited form as: Biochim Biophys Acta. 2011 Apr 15;1821(1):198–205. doi: 10.1016/j.bbalip.2011.04.004

Fig. 2.

Fig. 2

Deletion of entire mouse ADH gene family. The Adh-del strain was made possible by the fact that all six ADH genes in the mouse genome are located in a 250 kb stretch on chromosome 3 with no other genes intermixed [111]. Double-targeted embryonic stem (ES) cells were generated that had loxP sites introduced into both Adh4 and Adh3 located on opposite ends of the complex; double-targeted ES cells were selected for resistance to both neomycin (neo) and hygromycin (hygro). Mice generated from double-targeted ES cells were mated to a germ-line Cre to create homozygous Adh-del knockout mice lacking this 250 kb stretch containing the ADH gene family.