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. Author manuscript; available in PMC: 2012 May 24.
Published in final edited form as: Oncogene. 2011 May 23;30(47):4731–4739. doi: 10.1038/onc.2011.186

Figure 5. hPMC2 exonuclease activity is required for repair of estrogen-induced abasic sites.

Figure 5

MCF10A cells were transfected with either hPMC2 miRNA that targets hPMC2 3'-UTR or hPMC2 miRNA along with plasmid expressing WT or the DM hPMC2. Cells were either untreated or treated with 50 nM E2 for 24 hours. Genomic DNA was isolated from cells and processed for Aldehyde-Reactive Probe (ARP) labeling as described in “Materials and Methods”. Columns represent the number of AP sites/105 base pair (bp) in control or E2-treated samples. Error bars indicate standard deviation of 2 independent experiments. a, significance (P < 0.05) vs. untreated cells; b, significance (P < 0.05) vs. control transfected cells with the same treatment.