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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Prostate. 2011 May 20;72(3):233–243. doi: 10.1002/pros.21425

Figure 2.

Figure 2

Protein expression of PKCε, IL-6, PDK1, Akt and p-Akt (Ser473) in the prostrate of C57BL/6 mice fed with RD and HFD. (A) Total tissue extract were prepared as described in the Materials and Methods Section from the prostrate of mice fed with RD and HFD. The samples were subjected to SDS-PAGE gel electrophoresis. The blots were analyzed for the indicated antibodies and β-actin was used as the loading control. (B) Densitometric quantification represents changes in the levels of PKCε, PDK1, p-Akt (Ser473), and IL-6. White bars indicate RD, gray bars indicate HFD for 4 weeks and black bars indicate HFD for 8 weeks. Data are a mean of ± SD and corrected for loading. The single asterisk (*) indicates changes (p<0.05) and double asterisk (**) indicates the significant changes (p<0.001) in HFD compared to RD fed controls.