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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Prostate. 2011 May 20;72(3):233–243. doi: 10.1002/pros.21425

Figure 3.

Figure 3

Protein expression of Ser727 Stat-3, Tyr705 Stat-3, Stat-3, Ser536 p65, NF-κB/p65 in the nuclear fraction extracted from the prostrate of RD and HFD fed C57BL/6 mice. (A) Nuclear extract were prepared as described in the Materials and Methods Section from the prostrate of mice fed with RD and HFD. The samples were subjected to SDS-PAGE gel electrophoresis. The blots were analyzed for the indicated antibodies and Oct-1 was used as the loading control. (B) Densitometric quantification represents changes in the levels of Tyr705 Stat-3, Ser727 Stat-3, Stat-3, Ser536 p65, NF-κB/p65. White bars indicate RD, gray bars indicate HFD for 4 weeks and black bars indicate HFD for 8 weeks. Data are a mean of ± SD and corrected for loading. The double asterisk (**) indicates the significant changes (p<0.001) in HFD compared to RD fed controls.