Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
NF-κB and Stat-3 DNA-binding activity in the nuclear fraction extracted from the prostrate of RD and HFD fed C57BL/6 mice by EMSA. EMSA was performed to identify nuclear translocation of NF-κB and Stat-3 and their binding to DNA. The details are described in Materials and Methods Section. Controls: 1# biotin-EBNA control DNA; 2# biotin-EBNA control DNA + EBNA extract; and 3# biotin-EBNA control DNA + EBNA extract + 20-fold molar excess of unlabeled EBNA DNA.
