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Stat-3-κB DNA-binding activities in the nuclear fraction extracted from the prostrate of RD and HFD fed C57BL/6 mice by EMSA. EMSA was performed to identify interactions of Stat-3/NF-κB and their binding to DNA in the nuclear lysates. The details are described in Materials and Methods Section. Controls: 1# biotin-EBNA control DNA; 2# biotin-EBNA control DNA + EBNA extract; and 3# biotin-EBNA control DNA + EBNA extract + 20-fold molar excess of unlabeled EBNA DNA.
