Fig. 1.

KLK induces the accumulation of GPI-anchored proteins along the plasma membrane. T24 cells expressing GPI-hFR-mGFP were seeded on glass slides and analyzed by TIRF microscopy in the absence or presence of 100 μM KLK (A) or a mutant variant of KLK, termed KPK (B). (C) Two-color fluorescence microscopy experiments of the same cells additionally labeled with the fluorescent lipid tracer DiD. The incubation time of KLK or KPK was 5 min. Scale bars 20 μm (A), 25 μm (B), 10 μm (C).