Fig. 4.

KLK does not affect the localization of specific plasma membrane proteins. Experiments were performed using TIRF microscopy in the absence or presence of 100 μM KLK (5 min incubation time) on T24 cells expressing Caveolin-1-GFP (A), Lck-GFP (B), Fyn-YFP (C), Flotillin-1-GFP (D), Clathrin light chain-GFP (E), CD71-GFP (F), Orai1-GFP (G), CD147-YFP (H), SR-BI-GFP (I) or CD3ζ-YFP (J). Scale bars 30 (A), 35 (B), 10 (C), 30 (D), 20 (E), 30 (F), 20 (G), 10 (H), 20 (I) and 15 (J) μm, respectively.