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The KLK mediated re-localization of membrane-localized proteins is linked to a post-translational modification with sialic acids. HeLa cells expressing CD43-YFP were incubated with 1 U/ml neuraminidase to cleave sialic acids and treated afterwards with 100 μM KLK for 20 min. Pictures were taken 15 s, 5 min and 20 min after KLK addition. Scale bar 10 μm.
