Fig. 7.

KLK leads to an immobilization of the accumulated proteins. T24 cells expressing GPI-hFR-mGFP were treated with 100 μM KLK for 30 s and Fluorescence Recovery After Photobleaching (FRAP) experiments were performed. The mobile fraction within two defined regions (encircled region along the plasma membrane or region marked with a square below the cell) was recorded. Panel A indicates the fluorescence image before, panel B and C the fluorescence image 100 ms and 30 s after the bleach pulse, respectively. Scale bar 3 μm. The mobile fraction within the two regions is plotted in panel D and was calculated from measurements of ~ 15 individual cells. Error bars are based on the standard error of the mean.