Table 2.
CSFE+CD235a+ (%) | CSFE−CD235a + (%) | CSFE++CSFE−CD235a+−control (%) | Total no. of CD235+ cells/organ* | |
---|---|---|---|---|
BM | ||||
Control | 0.3 | 0.34 | 0 | |
Transfused mice (2 mice) | 0.53–0.75 | 1.1–1.07 | 0.99–1.18 | 3.0–3.5 × 106 |
Spleen | ||||
Control | 1.02 | 1.04 | 0 | |
Transfused mice (2 mice) | 3.34–5.75 | 1.3–1.6 | 2.57–5.29 | 2.6–5.3 × 106 |
The frequency of human CD235a+ cells was obtained after subtracting the background signal observed in untreated controls.
*The total number of cells per organ was calculated assuming that a mouse contain 300 × 106 BM and 100 × 106 spleen cells [19].