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. 2010 Jun 24;4:8. doi: 10.1186/1754-1611-4-8

Figure 2.

Figure 2

Protein synthesis as a function of plasmid concentration. (A) Luc synthesized as a function of plasmid pBEST-UTR1-Luc concentration in 3-PGA buffer (conditions: 0 mM magnesium glutamate, 80 mM potassium glutamate, 1.5 mM of each amino acids, 0.5% PEG 8000). (B) Kinetics of eGFP expression as a function of plasmid pBEST-UTR1-eGFP concentration in 3-PGA buffer (conditions: 4 mM magnesium glutamate, 60 mM potassium glutamate, 1 mM of each amino acids, 2.5% PEG 8000). Concentrations of magnesium glutamate and potassium glutamate reported here are the concentrations added to the cell-free reaction. The crude extract, dialyzed against the S30 buffer B, brings an additional 4.5 mM magnesium glutamate and 20 mM potassium glutamate to the cell-free reaction.