Table 4.
qPCR results comparing methods of template amplification
| Template | Itga5b 3' | Itga5b WT | Tmem8 3' | Tmem8 WT | Ankrd28 3' | Ankrd28 WT |
|---|---|---|---|---|---|---|
| Buccal 4 | 31.25 | 39.15 | 20.38 | 24.96 | 20.68 | 31.8 |
| Buccal 5 | 35.81 | No Ct | 20.36 | 25.31 | 20.92 | 31.36 |
| Blood 4 | 21.43 | 23.96 | 21.87 | 30.68 | 20.33 | 29.43 |
| Blood 5 | 20.56 | 23.9 | 21.11 | 30.23 | 20.22 | 29.82 |
| Control | 21.18 | 23.8 | 21.63 | 28.4 | 20.23 | 29.47 |
All amplifications were performed using either the Ovation V2 (3') or WT pico Nugen Kits, see Materials and Methods. Control RNA is from a pooled blood sample, see Materials and Methods.