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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: J Invest Dermatol. 2010 Jul 15;130(12):2773–2780. doi: 10.1038/jid.2010.209

Figure 1. Jacalin binds Dsg1.

Figure 1

(A) Binding to rDsg1. rDsg1 was incubated with jacalin-agarose (lanes 1, 3-6) or protein G/A-agarose beads (lane 2). Bound-Dsg1 was released by boiling in SDS sample buffer (lanes 1-2 and 5-6) or eluted with jacalin-inhibiting sugars (lane 3: 0.1 M melibiose; lane 4: 0.8 M D-galactose). In one set of experiment, jacalin beads were preincubated with 0.8 M D-galactose (lane 5) or buffer (lane 6) before adding the PF serum to further show the sugar-dependency binding. Released Dsg1 was analyzed by IB using anti-His antibodies. (B) Binding to epidermal Dsg1. Tissue lysates from human (H) or mouse (M) epidermis were incubated with jacalin-agarose (lanes 1 and 2) or protein G/A-agarose beads (lanes 3 and 4) followed by IB using anti-Dsg1 antibodies.