Table 1.

In vivo effect of plant lectins in IgG passive transfer mouse model of PF

IgG	Treatment	Oligosaccharide specificity	# mice tested	# mice with skin blister	# mice without skin blister
PF-1	Vehicle		15	15	0
	Jacalin	Galβ1-3GalNAc-α (asialo or sialo)	8	0	8
	PNA	GalNAc Galβ1-3GalNAc-α or -β (asialo)	9	1*	8
	VVL-B4	GalNAc	3	3	0
PF-2	Vehicle		6	6	0
	Jacalin		6	5**	1

Neonatal mice were preinjected (s.c.) with vehicle (TBS-Ca²⁺) or lectins for 2 h followed by pathogenic PF IgG injection (s.c.). Pathogenic IgG from two patients’ sera was used. PF-1 serum showed a remarkable inhibition in Dsgl binding by jacalin, whereas PF-2 showed a marginal reduction in Dsgl binding by jacalin. Animals were evaluated 20 h post PF IgG injection. Jacalin: 80 to 160 μg/g b.w.; PNA and VVL-B4: 160 μg/g b.w. Abbreviations: Gal, galactose; GalNAc, N-acetylglactosamine; Glc, glucose; PNA, peanut agglutinin; VVL-B4, vicia villosa-B4 lectin.

^*Minimum disease score (0.5+).

^**Overall less disease score (1.25 + 0.36) compared to those preinjected with vehicle (2.58 + 0.20) (p<0.01, Students’ t-test).