Figure 5.

Ranpirnase induces massive apoptosis in human mesothelioma (MM) xenografts. Immunohistochemical analysis of tissues from tumors grown in the peritoneal cavity of SCID mice and collected at necropsy. Parallel tissue sections were immunostained for human cytokeratin, as a marker of mesothelial origin, by the TUNEL apoptosis assay. The total number of cells and the number of TUNEL-positive apoptotic cells were determined by Image-J software on 8 different fields from 2 different sections. The percentages of apoptotic cells (±SD) are reported on the pictures of representative sections from vehicle- and ranpirnase (ONC)–treated animals.