Figure 8.

Silencing Gα₁₂ attenuates LPA-mediated SKOV3 cell migration. Proliferation of SKOV3, Hey, and OVCAR-3 cells (2.5 × 10⁵ cells/well) in which the expression of endogenous Gα₁₂ was silenced by stably expressed Gα₁₂-specific shRNA in response to LPA (20 µg) along with unstimulated control groups using crystal violet–based proliferation assay. Triplicate samples were collected at 0, 24, 48, and 72 hours following LPA stimulation and processed for analysis. Absorbance at 590 nm was used as an index of cell proliferation. Results are mean ± SEM from 3 independent experiments (upper panel). Silencing of endogenous Gα₁₂ by shRNA in the respective cell line was monitored by immunoblot analysis using Gα₁₂ antibodies (lower panel). The respective blots were stripped and reprobed with GAPDH antibodies to ascertain equal protein loading.