Fig. 3.

Both the constitutive CTMCs and the induced MMCs of the trachea express all seven secretory granule proteases. The Left column indicates the antiprotease specificity used to define the protease phenotype of tracheal MCs. Mice were either sensitized and not challenged (NC) or challenged (CH) for 7 d with analysis on day 4 postchallenge. Arrows indicate the protease reactive CTMCs in the submucosa of the trachea from NC and CH mice (Left and Right, respectively) and the induced intraepithelial tracheal MMCs from CH mice (Middle) that each expressed mMCP-1, -2, -4–7, and CPA3. No MMCs were found in the epithelium of trachea from NC mice. Images are representative of the results of analysis of three mice per group from three separate experiments. (Scale bar, 50 μm.)