Fig. 3.

Ahr deficiency inhibits Th17 cell differentiation in response to collagen immunization. (A and B) Fourteen days after the second immunization, sera were collected and levels of the proinflammatory cytokines IL-1β (A) and IL-6 (B) were measured by using specific ELISAs (WT, n = 8; Ahr KO, n = 6). (C) CD4⁺ T cells selected from inguinal lymph node cells by magnetic-activated cell sorting (MACS) obtained from mice 14 d after the second immunization were counted after intracellular staining of IFN-γ, IL-17, or Foxp3 (WT, n = 3; Ahr KO, n = 4; *P < 0.05; **P < 0.01).