Fig. 1.
Test of inducible full viral vector pICH18969. (A) Agroinfiltration for transient expression of vector pICH18969 (OD600 = 2.5 × 10-3) without (−) or with (+) the transcriptional activator AlcR (construct pICH18693; OD600 = 0.25). The leaf shown on the right was treated with ethanol 2 d after infiltration. (B) Leaf disc transformation with vectors pICH18969 and pICH18951. Leaf explants pictured 10 d after transformation. The same petri dish is shown under white light (right) and UV light (left). (C) Maps of the T-DNA regions of constructs pICH18969, pICH18951 and pICH18693. RB and LB, right and left borders; 35S-p, CaMV 35S promoter; alc-p, alcA promoter from Aspergillus nidulans; n-p and n-t, promoter and terminator of the Agrobacterium nopaline synthase gene; NPT, neomycin phosphotransferase II for selection of transgenic plants; RdRp, viral RNA-dependent RNA polymerase; MP, movement protein for cell-to-cell movement; 3′, viral 3′-nontranslated region; Ω, translational enhancer from TMV. Introns in the RdRp and MP are shown as white boxes. The hatched part of RdRp in construct pICH18951 indicates silent mutations.