Fig. 3.

Test of different ethanol treatments. Transgenic plants containing constructs pICH18693 (alcR) and pICH20592 (viral vector, MP) were treated with ethanol using different application methods: (A) noninduced plant; (B) one spray per day with 4% ethanol on five subsequent days; (C) same as (B) with two sprays per day; (D) root drenching with 4% ethanol; (E) root drenching repeated three times with 1% ethanol; (F) same as (E) with 2% ethanol; (G) root drenching and spraying with 4% ethanol; (H) incubation in ethanol vapor for 24 h without any pretreatment; (I) root drenching with 4% ethanol followed by incubation in ethanol vapor; and (J) spraying with 4% ethanol followed by incubation in ethanol vapor.