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. 2011 Aug 15;108(34):14330–14335. doi: 10.1073/pnas.1105172108

Fig. 1.

Fig. 1.

NO Enhances Cdk5 Kinase Activity. (A) Effect of SNOC on Cdk5/p35 phosphorylation of histone H1. HEK293 cells transfected with Cdk5 and p35 plasmids were exposed to 200 μM SNOC and cell lysates prepared for immunoblot analysis. Control cells were exposed to old SNOC, from which NO had been dissipated. The experiments were repeated four times. (B) Effect of SNOC on Cdk5 kinase activity. HEK293 cells transfected with Cdk5 and p35 plasmids were exposed to various concentrations of SNOC (50–200 μM) and cell lysates prepared for measurement of kinase activity using a luciferase assay. Control cells were treated with old SNOC. Kinase activity is normalized to control. Values are mean + SEM (n > 3 for each group, **P < 0.01 by ANOVA). (C) SNOC enhances Cdk5/p25 activity, monitored by histone H1 phosphorylation. HEK293 cells transfected with Cdk5 and p25 plasmids were exposed to 200 μM SNOC and cell lysates prepared for immunoblot analysis. Control cells were exposed to old SNOC.