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. 2011 Aug 15;108(34):14228–14233. doi: 10.1073/pnas.1108809108

Fig. 5.

Fig. 5.

CD1e expressed in Tg DCs influences presentation of self-lipids. (A) DCs from Tg mice (●) or non-Tg littermates (○) were incubated for different lengths of time with α-GalCer (10 ng/mL) and then fixed before addition of iNKT mouse hybridoma cells. (B) The half-life of CD1d–α-GalCer complexes is reduced in Tg DCs. DCs from Tg mice (●) or non-Tg littermates (○) were pulsed for 2 h with α-GalCer (2 ng/mL) and chased for different lengths of time before addition of iNKT mouse hybridoma cells. (A and B) Released mIL-2 (ng/mL ± SD) was determined in triplicate. (C) Detection of CD1d–α-GalCer complexes by L363 antibody staining on the surface of Tg (●) or WT (○) DCs incubated for 24 h with α-GalCer. (D) Detection of CD1d–α-GalCer complexes by L363 antibody on Tg (●) or WT (○) DCs pulsed for 24 h with different doses of α-GalCer and chased for 24 h before staining. Data shown are representative of three independent experiments.