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Characterization of peptide binding to column-bound heparin. (A) Elution profiles for each peptide as the NaCl concentration increased from 0 to 2 M. Column retention of each peptide was linearly proportional to the total (B) and net (C) number of positive charges. (B) ○, p1; ●, p6; □, p5; ◊, p3; ▪, p7; △, p4; ▲, p2. (C) ○, p1; ●, p6; □, p3; ▪, p5; △, p4; ▲, p7.
