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. 2011 Aug 22;6(8):e23388. doi: 10.1371/journal.pone.0023388

Figure 7. Inhibition of HIV-1 replication by nano-curcumin is due to abolished viral cDNA synthesis and/or altered topology.

Figure 7

SUPT1 cells were challenged for 4 h with HIV-193IN101 in the presence of 5 µM of sol-curcumin, nano-curcumin or nano-apotransferrin. A) The expression of topoisomerase IIα was determined by (i) semi-quantitative and (ii) quantitative-PCR. B) Quantity of viral cDNA synthesized was shown by both (i) semi-quantitative and (ii) real-time PCR using gag-specific primers. Template from normal SUP-T1 cells was used as negative control. Azidothymidine (AZT) was employed as a positive control and 18S was used as an internal control in both experiments. Error bars indicate SD. ***, P≤0.001 compared to sol-curcumin.